diff --git a/cmd/evm/internal/t8ntool/transition.go b/cmd/evm/internal/t8ntool/transition.go
index af60333cbd..1226656eb9 100644
--- a/cmd/evm/internal/t8ntool/transition.go
+++ b/cmd/evm/internal/t8ntool/transition.go
@@ -428,7 +428,7 @@ func BinKeys(ctx *cli.Context) error {
 	db := triedb.NewDatabase(rawdb.NewMemoryDatabase(), triedb.VerkleDefaults)
 	defer db.Close()
 
-	bt, err := genBinTrieFromAlloc(alloc, db)
+	bt, err := genBinTrieFromAlloc(alloc, db, db.BinTrieGroupDepth())
 	if err != nil {
 		return fmt.Errorf("error generating bt: %w", err)
 	}
@@ -472,7 +472,7 @@ func BinTrieRoot(ctx *cli.Context) error {
 	db := triedb.NewDatabase(rawdb.NewMemoryDatabase(), triedb.VerkleDefaults)
 	defer db.Close()
 
-	bt, err := genBinTrieFromAlloc(alloc, db)
+	bt, err := genBinTrieFromAlloc(alloc, db, db.BinTrieGroupDepth())
 	if err != nil {
 		return fmt.Errorf("error generating bt: %w", err)
 	}
@@ -482,8 +482,8 @@ func BinTrieRoot(ctx *cli.Context) error {
 }
 
 // TODO(@CPerezz): Should this go to `bintrie` module?
-func genBinTrieFromAlloc(alloc core.GenesisAlloc, db database.NodeDatabase) (*bintrie.BinaryTrie, error) {
-	bt, err := bintrie.NewBinaryTrie(types.EmptyBinaryHash, db)
+func genBinTrieFromAlloc(alloc core.GenesisAlloc, db database.NodeDatabase, groupDepth int) (*bintrie.BinaryTrie, error) {
+	bt, err := bintrie.NewBinaryTrie(types.EmptyBinaryHash, db, groupDepth)
 	if err != nil {
 		return nil, err
 	}
diff --git a/cmd/utils/flags.go b/cmd/utils/flags.go
index 844397b734..6775dce676 100644
--- a/cmd/utils/flags.go
+++ b/cmd/utils/flags.go
@@ -289,6 +289,12 @@ var (
 		Value:    ethconfig.Defaults.EnableStateSizeTracking,
 		Category: flags.StateCategory,
 	}
+	BinTrieGroupDepthFlag = &cli.IntFlag{
+		Name:     "bintrie.groupdepth",
+		Usage:    "Number of levels per serialized group in binary trie (1-8, default 8). Lower values create smaller groups with more nodes.",
+		Value:    8,
+		Category: flags.StateCategory,
+	}
 	StateHistoryFlag = &cli.Uint64Flag{
 		Name:     "history.state",
 		Usage:    "Number of recent blocks to retain state history for, only relevant in state.scheme=path (default = 90,000 blocks, 0 = entire chain)",
@@ -1720,9 +1726,6 @@ func SetEthConfig(ctx *cli.Context, stack *node.Node, cfg *ethconfig.Config) {
 	if ctx.IsSet(TrienodeHistoryFlag.Name) {
 		cfg.TrienodeHistory = ctx.Int64(TrienodeHistoryFlag.Name)
 	}
-	if ctx.IsSet(TrienodeHistoryFullValueCheckpointFlag.Name) {
-		cfg.NodeFullValueCheckpoint = uint32(ctx.Uint(TrienodeHistoryFullValueCheckpointFlag.Name))
-	}
 	if ctx.IsSet(StateSchemeFlag.Name) {
 		cfg.StateScheme = ctx.String(StateSchemeFlag.Name)
 	}
@@ -2338,6 +2341,7 @@ func MakeChain(ctx *cli.Context, stack *node.Node, readonly bool) (*core.BlockCh
 		StateHistory:            ctx.Uint64(StateHistoryFlag.Name),
 		TrienodeHistory:         ctx.Int64(TrienodeHistoryFlag.Name),
 		NodeFullValueCheckpoint: uint32(ctx.Uint(TrienodeHistoryFullValueCheckpointFlag.Name)),
+		BinTrieGroupDepth:       ctx.Int(BinTrieGroupDepthFlag.Name),
 
 		// Disable transaction indexing/unindexing.
 		TxLookupLimit: -1,
diff --git a/core/blockchain.go b/core/blockchain.go
index 8741b8b937..b7329cad66 100644
--- a/core/blockchain.go
+++ b/core/blockchain.go
@@ -169,9 +169,10 @@ type BlockChainConfig struct {
 	TrieNoAsyncFlush     bool          // Whether the asynchronous buffer flushing is disallowed
 	TrieJournalDirectory string        // Directory path to the journal used for persisting trie data across node restarts
 
-	Preimages   bool   // Whether to store preimage of trie key to the disk
-	StateScheme string // Scheme used to store ethereum states and merkle tree nodes on top
-	ArchiveMode bool   // Whether to enable the archive mode
+	Preimages         bool   // Whether to store preimage of trie key to the disk
+	StateScheme       string // Scheme used to store ethereum states and merkle tree nodes on top
+	ArchiveMode       bool   // Whether to enable the archive mode
+	BinTrieGroupDepth int    // Number of levels per serialized group in binary trie (1-8)
 
 	// Number of blocks from the chain head for which state histories are retained.
 	// If set to 0, all state histories across the entire chain will be retained;
@@ -255,8 +256,9 @@ func (cfg BlockChainConfig) WithNoAsyncFlush(on bool) *BlockChainConfig {
 // triedbConfig derives the configures for trie database.
 func (cfg *BlockChainConfig) triedbConfig(isVerkle bool) *triedb.Config {
 	config := &triedb.Config{
-		Preimages: cfg.Preimages,
-		IsVerkle:  isVerkle,
+		Preimages:        cfg.Preimages,
+		IsVerkle:         isVerkle,
+		BinTrieGroupDepth: cfg.BinTrieGroupDepth,
 	}
 	if cfg.StateScheme == rawdb.HashScheme {
 		config.HashDB = &hashdb.Config{
diff --git a/core/state/database.go b/core/state/database.go
index 4a5547d075..82e585ff57 100644
--- a/core/state/database.go
+++ b/core/state/database.go
@@ -246,7 +246,7 @@ func (db *CachingDB) OpenTrie(root common.Hash) (Trie, error) {
 		if ts.Transitioned() {
 			// Use BinaryTrie instead of VerkleTrie when IsVerkle is set
 			// (IsVerkle actually means Binary Trie mode in this codebase)
-			return bintrie.NewBinaryTrie(root, db.triedb)
+			return bintrie.NewBinaryTrie(root, db.triedb, db.triedb.BinTrieGroupDepth())
 		}
 	}
 	tr, err := trie.NewStateTrie(trie.StateTrieID(root), db.triedb)
diff --git a/core/state/reader.go b/core/state/reader.go
index 2db9d1f9b4..0d094030d9 100644
--- a/core/state/reader.go
+++ b/core/state/reader.go
@@ -302,7 +302,7 @@ func newTrieReader(root common.Hash, db *triedb.Database) (*trieReader, error) {
 		tr, err = trie.NewStateTrie(trie.StateTrieID(root), db)
 	} else {
 		// When IsVerkle() is true, create a BinaryTrie wrapped in TransitionTrie
-		binTrie, binErr := bintrie.NewBinaryTrie(root, db)
+		binTrie, binErr := bintrie.NewBinaryTrie(root, db, db.BinTrieGroupDepth())
 		if binErr != nil {
 			return nil, binErr
 		}
diff --git a/eth/backend.go b/eth/backend.go
index aed1542aeb..d765a663e9 100644
--- a/eth/backend.go
+++ b/eth/backend.go
@@ -232,6 +232,7 @@ func New(stack *node.Node, config *ethconfig.Config) (*Ethereum, error) {
 			StateHistory:            config.StateHistory,
 			TrienodeHistory:         config.TrienodeHistory,
 			NodeFullValueCheckpoint: config.NodeFullValueCheckpoint,
+			BinTrieGroupDepth:       config.BinTrieGroupDepth,
 			StateScheme:             scheme,
 			ChainHistoryMode:        config.HistoryMode,
 			TxLookupLimit:           int64(min(config.TransactionHistory, math.MaxInt64)),
diff --git a/eth/ethconfig/config.go b/eth/ethconfig/config.go
index e58c4b884a..1505ce6524 100644
--- a/eth/ethconfig/config.go
+++ b/eth/ethconfig/config.go
@@ -59,6 +59,7 @@ var Defaults = Config{
 	StateHistory:            pathdb.Defaults.StateHistory,
 	TrienodeHistory:         pathdb.Defaults.TrienodeHistory,
 	NodeFullValueCheckpoint: pathdb.Defaults.FullValueCheckpoint,
+	BinTrieGroupDepth:       8, // byte-aligned groups by default
 	DatabaseCache:           512,
 	TrieCleanCache:          154,
 	TrieDirtyCache:          256,
@@ -125,6 +126,11 @@ type Config struct {
 	// consistent with persistent state.
 	StateScheme string `toml:",omitempty"`
 
+	// BinTrieGroupDepth is the number of levels per serialized group in binary trie.
+	// Valid values are 1-8, with 8 being the default (byte-aligned groups).
+	// Lower values create smaller groups with more nodes.
+	BinTrieGroupDepth int `toml:",omitempty"`
+
 	// RequiredBlocks is a set of block number -> hash mappings which must be in the
 	// canonical chain of all remote peers. Setting the option makes geth verify the
 	// presence of these blocks for every new peer connection.
diff --git a/eth/ethconfig/gen_config.go b/eth/ethconfig/gen_config.go
index 6f94a409e5..09e1ee1e78 100644
--- a/eth/ethconfig/gen_config.go
+++ b/eth/ethconfig/gen_config.go
@@ -34,6 +34,7 @@ func (c Config) MarshalTOML() (interface{}, error) {
 		TrienodeHistory         int64                  `toml:",omitempty"`
 		NodeFullValueCheckpoint uint32                 `toml:",omitempty"`
 		StateScheme             string                 `toml:",omitempty"`
+		BinTrieGroupDepth       int                    `toml:",omitempty"`
 		RequiredBlocks          map[uint64]common.Hash `toml:"-"`
 		SlowBlockThreshold      time.Duration          `toml:",omitempty"`
 		SkipBcVersionCheck      bool                   `toml:"-"`
@@ -87,6 +88,7 @@ func (c Config) MarshalTOML() (interface{}, error) {
 	enc.TrienodeHistory = c.TrienodeHistory
 	enc.NodeFullValueCheckpoint = c.NodeFullValueCheckpoint
 	enc.StateScheme = c.StateScheme
+	enc.BinTrieGroupDepth = c.BinTrieGroupDepth
 	enc.RequiredBlocks = c.RequiredBlocks
 	enc.SlowBlockThreshold = c.SlowBlockThreshold
 	enc.SkipBcVersionCheck = c.SkipBcVersionCheck
@@ -144,6 +146,7 @@ func (c *Config) UnmarshalTOML(unmarshal func(interface{}) error) error {
 		TrienodeHistory         *int64                 `toml:",omitempty"`
 		NodeFullValueCheckpoint *uint32                `toml:",omitempty"`
 		StateScheme             *string                `toml:",omitempty"`
+		BinTrieGroupDepth       *int                   `toml:",omitempty"`
 		RequiredBlocks          map[uint64]common.Hash `toml:"-"`
 		SlowBlockThreshold      *time.Duration         `toml:",omitempty"`
 		SkipBcVersionCheck      *bool                  `toml:"-"`
@@ -234,6 +237,9 @@ func (c *Config) UnmarshalTOML(unmarshal func(interface{}) error) error {
 	if dec.StateScheme != nil {
 		c.StateScheme = *dec.StateScheme
 	}
+	if dec.BinTrieGroupDepth != nil {
+		c.BinTrieGroupDepth = *dec.BinTrieGroupDepth
+	}
 	if dec.RequiredBlocks != nil {
 		c.RequiredBlocks = dec.RequiredBlocks
 	}
diff --git a/trie/bintrie/binary_node.go b/trie/bintrie/binary_node.go
index e1eee7a7bd..07a9107642 100644
--- a/trie/bintrie/binary_node.go
+++ b/trie/bintrie/binary_node.go
@@ -31,21 +31,30 @@ type (
 var zero [32]byte
 
 const (
-	StemNodeWidth = 256 // Number of child per leaf node
-	StemSize      = 31  // Number of bytes to travel before reaching a group of leaves
-	NodeTypeBytes = 1   // Size of node type prefix in serialization
-	HashSize      = 32  // Size of a hash in bytes
-	BitmapSize    = 32  // Size of the bitmap in a stem node
+	StemNodeWidth  = 256 // Number of child per leaf node
+	StemSize       = 31  // Number of bytes to travel before reaching a group of leaves
+	NodeTypeBytes  = 1   // Size of node type prefix in serialization
+	HashSize       = 32  // Size of a hash in bytes
+	StemBitmapSize = 32  // Size of the bitmap in a stem node (256 values = 32 bytes)
 
-	// GroupDepth is the number of levels in a grouped subtree serialization.
-	// Groups are byte-aligned (depth % 8 == 0). This may become configurable later.
+	// MaxGroupDepth is the maximum allowed group depth for InternalNode serialization.
+	// Valid group depths are 1-8, where depth N means 2^N bottom-layer positions.
 	// Serialization format for InternalNode groups:
-	//   [1 byte type] [1 byte group depth (1-8)] [32 byte bitmap] [N × 32 byte hashes]
-	// The bitmap has 2^groupDepth bits, indicating which bottom-layer children are present.
-	// Only present children's hashes are stored, in order.
-	GroupDepth = 8
+	//   [1 byte type] [1 byte group depth (1-8)] [variable bitmap] [N × 32 byte hashes]
+	// The bitmap has 2^groupDepth bits (BitmapSizeForDepth bytes), indicating which
+	// bottom-layer children are present. Only present children's hashes are stored.
+	MaxGroupDepth = 8
 )
 
+// BitmapSizeForDepth returns the bitmap size in bytes for a given group depth.
+// For depths 1-3, returns 1 byte. For depths 4-8, returns 2^(depth-3) bytes.
+func BitmapSizeForDepth(groupDepth int) int {
+	if groupDepth <= 3 {
+		return 1
+	}
+	return 1 << (groupDepth - 3)
+}
+
 const (
 	nodeTypeStem = iota + 1 // Stem node, contains a stem and a bitmap of values
 	nodeTypeInternal
@@ -59,7 +68,7 @@ type BinaryNode interface {
 	Hash() common.Hash
 	GetValuesAtStem([]byte, NodeResolverFn) ([][]byte, error)
 	InsertValuesAtStem([]byte, [][]byte, NodeResolverFn, int) (BinaryNode, error)
-	CollectNodes([]byte, NodeFlushFn) error
+	CollectNodes([]byte, NodeFlushFn, int) error // groupDepth parameter for serialization
 
 	toDot(parent, path string) string
 	GetHeight() int
@@ -106,25 +115,28 @@ func serializeSubtree(node BinaryNode, remainingDepth int, position int, bitmap
 }
 
 // SerializeNode serializes a binary trie node into a byte slice.
-func SerializeNode(node BinaryNode) []byte {
+// groupDepth specifies how many levels to include in an InternalNode group (1-8).
+func SerializeNode(node BinaryNode, groupDepth int) []byte {
+	if groupDepth < 1 || groupDepth > MaxGroupDepth {
+		panic("groupDepth must be between 1 and 8")
+	}
 	switch n := (node).(type) {
 	case *InternalNode:
-		// InternalNode group: 1 byte type + 1 byte group depth + 32 byte bitmap + N×32 byte hashes
-		groupDepth := GroupDepth
-
-		var bitmap [BitmapSize]byte
+		// InternalNode group: 1 byte type + 1 byte group depth + variable bitmap + N×32 byte hashes
+		bitmapSize := BitmapSizeForDepth(groupDepth)
+		bitmap := make([]byte, bitmapSize)
 		var hashes []common.Hash
 
-		serializeSubtree(n, groupDepth, 0, bitmap[:], &hashes)
+		serializeSubtree(n, groupDepth, 0, bitmap, &hashes)
 
 		// Build serialized output
-		serializedLen := NodeTypeBytes + 1 + BitmapSize + len(hashes)*HashSize
+		serializedLen := NodeTypeBytes + 1 + bitmapSize + len(hashes)*HashSize
 		serialized := make([]byte, serializedLen)
 		serialized[0] = nodeTypeInternal
 		serialized[1] = byte(groupDepth)
-		copy(serialized[2:2+BitmapSize], bitmap[:])
+		copy(serialized[2:2+bitmapSize], bitmap)
 
-		offset := NodeTypeBytes + 1 + BitmapSize
+		offset := NodeTypeBytes + 1 + bitmapSize
 		for _, h := range hashes {
 			copy(serialized[offset:offset+HashSize], h.Bytes())
 			offset += HashSize
@@ -207,16 +219,20 @@ func DeserializeNode(serialized []byte, depth int) (BinaryNode, error) {
 
 	switch serialized[0] {
 	case nodeTypeInternal:
-		// Grouped format: 1 byte type + 1 byte group depth + 32 byte bitmap + N×32 byte hashes
-		if len(serialized) < NodeTypeBytes+1+BitmapSize {
+		// Grouped format: 1 byte type + 1 byte group depth + variable bitmap + N×32 byte hashes
+		if len(serialized) < NodeTypeBytes+1 {
 			return nil, invalidSerializedLength
 		}
 		groupDepth := int(serialized[1])
-		if groupDepth < 1 || groupDepth > GroupDepth {
+		if groupDepth < 1 || groupDepth > MaxGroupDepth {
 			return nil, errors.New("invalid group depth")
 		}
-		bitmap := serialized[2 : 2+BitmapSize]
-		hashData := serialized[2+BitmapSize:]
+		bitmapSize := BitmapSizeForDepth(groupDepth)
+		if len(serialized) < NodeTypeBytes+1+bitmapSize {
+			return nil, invalidSerializedLength
+		}
+		bitmap := serialized[2 : 2+bitmapSize]
+		hashData := serialized[2+bitmapSize:]
 
 		// Count present children from bitmap
 		hashIdx := 0
diff --git a/trie/bintrie/binary_node_test.go b/trie/bintrie/binary_node_test.go
index a04f08c9b3..2fe068d869 100644
--- a/trie/bintrie/binary_node_test.go
+++ b/trie/bintrie/binary_node_test.go
@@ -37,27 +37,28 @@ func TestSerializeDeserializeInternalNode(t *testing.T) {
 		right: HashedNode(rightHash),
 	}
 
-	// Serialize the node
-	serialized := SerializeNode(node)
+	// Serialize the node with default group depth of 8
+	serialized := SerializeNode(node, MaxGroupDepth)
 
 	// Check the serialized format: type byte + group depth byte + 32 byte bitmap + N*32 byte hashes
 	if serialized[0] != nodeTypeInternal {
 		t.Errorf("Expected type byte to be %d, got %d", nodeTypeInternal, serialized[0])
 	}
 
-	if serialized[1] != GroupDepth {
-		t.Errorf("Expected group depth to be %d, got %d", GroupDepth, serialized[1])
+	if serialized[1] != MaxGroupDepth {
+		t.Errorf("Expected group depth to be %d, got %d", MaxGroupDepth, serialized[1])
 	}
 
 	// Expected length: 1 (type) + 1 (group depth) + 32 (bitmap) + 2*32 (two hashes) = 98 bytes
-	expectedLen := NodeTypeBytes + 1 + BitmapSize + 2*HashSize
+	bitmapSize := BitmapSizeForDepth(MaxGroupDepth)
+	expectedLen := NodeTypeBytes + 1 + bitmapSize + 2*HashSize
 	if len(serialized) != expectedLen {
 		t.Errorf("Expected serialized length to be %d, got %d", expectedLen, len(serialized))
 	}
 
 	// The left child (HashedNode) terminates at remainingDepth=7, so it's placed at position 0<<7 = 0
 	// The right child (HashedNode) terminates at remainingDepth=7, so it's placed at position 1<<7 = 128
-	bitmap := serialized[2 : 2+BitmapSize]
+	bitmap := serialized[2 : 2+bitmapSize]
 	if bitmap[0]&0x80 == 0 { // bit 0 (MSB of byte 0)
 		t.Error("Expected bit 0 to be set in bitmap (left child)")
 	}
@@ -135,8 +136,8 @@ func TestSerializeDeserializeStemNode(t *testing.T) {
 		depth:  10,
 	}
 
-	// Serialize the node
-	serialized := SerializeNode(node)
+	// Serialize the node (groupDepth doesn't affect StemNode serialization)
+	serialized := SerializeNode(node, MaxGroupDepth)
 
 	// Check the serialized format
 	if serialized[0] != nodeTypeStem {
@@ -214,8 +215,8 @@ func TestDeserializeInvalidType(t *testing.T) {
 
 // TestDeserializeInvalidLength tests deserialization with invalid data length
 func TestDeserializeInvalidLength(t *testing.T) {
-	// InternalNode with type byte 1 but wrong length
-	invalidData := []byte{nodeTypeInternal, 0, 0} // Too short for internal node
+	// InternalNode with valid type byte and group depth but too short for bitmap
+	invalidData := []byte{nodeTypeInternal, 8, 0, 0} // Too short for bitmap (needs 32 bytes)
 
 	_, err := DeserializeNode(invalidData, 0)
 	if err == nil {
diff --git a/trie/bintrie/empty.go b/trie/bintrie/empty.go
index 7cfe373b35..7d426e15bf 100644
--- a/trie/bintrie/empty.go
+++ b/trie/bintrie/empty.go
@@ -59,7 +59,7 @@ func (e Empty) InsertValuesAtStem(key []byte, values [][]byte, _ NodeResolverFn,
 	}, nil
 }
 
-func (e Empty) CollectNodes(_ []byte, _ NodeFlushFn) error {
+func (e Empty) CollectNodes(_ []byte, _ NodeFlushFn, _ int) error {
 	return nil
 }
 
diff --git a/trie/bintrie/empty_test.go b/trie/bintrie/empty_test.go
index 574ae1830b..07dcefcaff 100644
--- a/trie/bintrie/empty_test.go
+++ b/trie/bintrie/empty_test.go
@@ -186,7 +186,7 @@ func TestEmptyCollectNodes(t *testing.T) {
 		collected = append(collected, n)
 	}
 
-	err := node.CollectNodes([]byte{0, 1, 0}, flushFn)
+	err := node.CollectNodes([]byte{0, 1, 0}, flushFn, MaxGroupDepth)
 	if err != nil {
 		t.Fatalf("Unexpected error: %v", err)
 	}
diff --git a/trie/bintrie/group_debug_test.go b/trie/bintrie/group_debug_test.go
index cc54a6f68c..87a8d6ea43 100644
--- a/trie/bintrie/group_debug_test.go
+++ b/trie/bintrie/group_debug_test.go
@@ -18,11 +18,12 @@ func TestGroupedSerializationDebug(t *testing.T) {
 		right: HashedNode(rightHash),
 	}
 
-	serialized := SerializeNode(node)
+	serialized := SerializeNode(node, MaxGroupDepth)
 	t.Logf("Serialized length: %d", len(serialized))
 	t.Logf("Type: %d, GroupDepth: %d", serialized[0], serialized[1])
 
-	bitmap := serialized[2 : 2+BitmapSize]
+	bitmapSize := BitmapSizeForDepth(MaxGroupDepth)
+	bitmap := serialized[2 : 2+bitmapSize]
 	t.Logf("Bitmap: %x", bitmap)
 
 	// Count and show set bits
@@ -69,12 +70,13 @@ func TestFullDepth8Tree(t *testing.T) {
 	// Build a full 8-level tree
 	root := buildFullTree(0, 8)
 
-	serialized := SerializeNode(root)
+	serialized := SerializeNode(root, MaxGroupDepth)
 	t.Logf("Full tree serialized length: %d", len(serialized))
 	t.Logf("Expected: 1 + 1 + 32 + 256*32 = %d", 1+1+32+256*32)
 
 	// Count set bits in bitmap
-	bitmap := serialized[2 : 2+BitmapSize]
+	bitmapSize := BitmapSizeForDepth(MaxGroupDepth)
+	bitmap := serialized[2 : 2+bitmapSize]
 	count := 0
 	for i := 0; i < 256; i++ {
 		if bitmap[i/8]>>(7-(i%8))&1 == 1 {
@@ -146,7 +148,7 @@ func TestRoundTripPreservesHashes(t *testing.T) {
 
 	root := buildTreeWithHashes(0, 8, 0, hashes)
 
-	serialized := SerializeNode(root)
+	serialized := SerializeNode(root, MaxGroupDepth)
 	deserialized, err := DeserializeNode(serialized, 0)
 	if err != nil {
 		t.Fatalf("Error: %v", err)
@@ -205,9 +207,9 @@ func TestCollectNodesGrouping(t *testing.T) {
 		}{pathCopy, node})
 
 		// Serialize and store by hash
-		serialized := SerializeNode(node)
+		serialized := SerializeNode(node, MaxGroupDepth)
 		serializedNodes[node.Hash()] = serialized
-	})
+	}, MaxGroupDepth)
 	if err != nil {
 		t.Fatalf("CollectNodes failed: %v", err)
 	}
@@ -362,3 +364,64 @@ func buildDeepTreeUnique(depth, maxDepth, position int) BinaryNode {
 		right: buildDeepTreeUnique(depth+1, maxDepth, position*2+1),
 	}
 }
+
+// TestVariableGroupDepth tests serialization with different group depths (1-8)
+func TestVariableGroupDepth(t *testing.T) {
+	for groupDepth := 1; groupDepth <= MaxGroupDepth; groupDepth++ {
+		t.Run(fmt.Sprintf("groupDepth=%d", groupDepth), func(t *testing.T) {
+			// Build a tree with depth equal to groupDepth * 2 (two full groups)
+			treeDepth := groupDepth * 2
+			root := buildDeepTreeUnique(0, treeDepth, 0)
+			originalHash := root.Hash()
+
+			// Serialize with this group depth
+			serialized := SerializeNode(root, groupDepth)
+
+			// Verify header
+			if serialized[0] != nodeTypeInternal {
+				t.Errorf("Expected type byte %d, got %d", nodeTypeInternal, serialized[0])
+			}
+			if int(serialized[1]) != groupDepth {
+				t.Errorf("Expected group depth %d, got %d", groupDepth, serialized[1])
+			}
+
+			// Verify bitmap size
+			expectedBitmapSize := BitmapSizeForDepth(groupDepth)
+			expectedMinLen := 1 + 1 + expectedBitmapSize // type + depth + bitmap
+			if len(serialized) < expectedMinLen {
+				t.Errorf("Serialized data too short: got %d, expected at least %d", len(serialized), expectedMinLen)
+			}
+
+			// Deserialize and verify hash matches
+			deserialized, err := DeserializeNode(serialized, 0)
+			if err != nil {
+				t.Fatalf("DeserializeNode failed: %v", err)
+			}
+
+			if deserialized.Hash() != originalHash {
+				t.Errorf("Hash mismatch after round-trip: expected %x, got %x", originalHash, deserialized.Hash())
+			}
+
+			// Collect nodes and verify correct grouping
+			var collectedDepths []int
+			err = root.CollectNodes(nil, func(path []byte, node BinaryNode) {
+				if in, ok := node.(*InternalNode); ok {
+					collectedDepths = append(collectedDepths, in.depth)
+				}
+			}, groupDepth)
+			if err != nil {
+				t.Fatalf("CollectNodes failed: %v", err)
+			}
+
+			// Verify all collected nodes are at group boundaries
+			for _, depth := range collectedDepths {
+				if depth%groupDepth != 0 {
+					t.Errorf("Collected node at depth %d, but groupDepth is %d (not a boundary)", depth, groupDepth)
+				}
+			}
+
+			t.Logf("groupDepth=%d: serialized=%d bytes, collected=%d nodes at depths %v",
+				groupDepth, len(serialized), len(collectedDepths), collectedDepths)
+		})
+	}
+}
diff --git a/trie/bintrie/hashed_node.go b/trie/bintrie/hashed_node.go
index e4d8c2e7ac..a3b884ecdb 100644
--- a/trie/bintrie/hashed_node.go
+++ b/trie/bintrie/hashed_node.go
@@ -80,7 +80,7 @@ func (h HashedNode) toDot(parent string, path string) string {
 	return ret
 }
 
-func (h HashedNode) CollectNodes([]byte, NodeFlushFn) error {
+func (h HashedNode) CollectNodes([]byte, NodeFlushFn, int) error {
 	// HashedNodes are already persisted in the database and don't need to be collected.
 	return nil
 }
diff --git a/trie/bintrie/hashed_node_test.go b/trie/bintrie/hashed_node_test.go
index f9e6984888..9fba61bdff 100644
--- a/trie/bintrie/hashed_node_test.go
+++ b/trie/bintrie/hashed_node_test.go
@@ -135,7 +135,7 @@ func TestHashedNodeInsertValuesAtStem(t *testing.T) {
 	}
 
 	// Serialize the node
-	serialized := SerializeNode(originalNode)
+	serialized := SerializeNode(originalNode, MaxGroupDepth)
 
 	// Create a mock resolver that returns the serialized node
 	validResolver := func(path []byte, hash common.Hash) ([]byte, error) {
diff --git a/trie/bintrie/internal_node.go b/trie/bintrie/internal_node.go
index 6351731fe0..9fcb8818d8 100644
--- a/trie/bintrie/internal_node.go
+++ b/trie/bintrie/internal_node.go
@@ -184,15 +184,18 @@ func (bt *InternalNode) InsertValuesAtStem(stem []byte, values [][]byte, resolve
 	return bt, err
 }
 
-// CollectNodes collects all child nodes at group boundaries (every GroupDepth levels),
-// and flushes them into the provided node collector. Each flush serializes an 8-level
+// CollectNodes collects all child nodes at group boundaries (every groupDepth levels),
+// and flushes them into the provided node collector. Each flush serializes a groupDepth-level
 // subtree group. Nodes within a group are not flushed individually.
-func (bt *InternalNode) CollectNodes(path []byte, flushfn NodeFlushFn) error {
-	// Only flush at group boundaries (depth % GroupDepth == 0)
-	if bt.depth%GroupDepth == 0 {
+func (bt *InternalNode) CollectNodes(path []byte, flushfn NodeFlushFn, groupDepth int) error {
+	if groupDepth < 1 || groupDepth > MaxGroupDepth {
+		return errors.New("groupDepth must be between 1 and 8")
+	}
+	// Only flush at group boundaries (depth % groupDepth == 0)
+	if bt.depth%groupDepth == 0 {
 		// We're at a group boundary - first collect any nodes in deeper groups,
 		// then flush this group
-		if err := bt.collectChildGroups(path, flushfn, GroupDepth-1); err != nil {
+		if err := bt.collectChildGroups(path, flushfn, groupDepth, groupDepth-1); err != nil {
 			return err
 		}
 		flushfn(path, bt)
@@ -200,22 +203,22 @@ func (bt *InternalNode) CollectNodes(path []byte, flushfn NodeFlushFn) error {
 	}
 	// Not at a group boundary - this shouldn't happen if we're called correctly from root
 	// but handle it by continuing to traverse
-	return bt.collectChildGroups(path, flushfn, GroupDepth-(bt.depth%GroupDepth)-1)
+	return bt.collectChildGroups(path, flushfn, groupDepth, groupDepth-(bt.depth%groupDepth)-1)
 }
 
 // collectChildGroups traverses within a group to find and collect nodes in the next group.
 // remainingLevels is how many more levels below the current node until we reach the group boundary.
 // When remainingLevels=0, the current node's children are at the next group boundary.
-func (bt *InternalNode) collectChildGroups(path []byte, flushfn NodeFlushFn, remainingLevels int) error {
+func (bt *InternalNode) collectChildGroups(path []byte, flushfn NodeFlushFn, groupDepth int, remainingLevels int) error {
 	if remainingLevels == 0 {
 		// Current node is at depth (groupBoundary - 1), its children are at the next group boundary
 		if bt.left != nil {
-			if err := bt.left.CollectNodes(appendBit(path, 0), flushfn); err != nil {
+			if err := bt.left.CollectNodes(appendBit(path, 0), flushfn, groupDepth); err != nil {
 				return err
 			}
 		}
 		if bt.right != nil {
-			if err := bt.right.CollectNodes(appendBit(path, 1), flushfn); err != nil {
+			if err := bt.right.CollectNodes(appendBit(path, 1), flushfn, groupDepth); err != nil {
 				return err
 			}
 		}
@@ -226,12 +229,12 @@ func (bt *InternalNode) collectChildGroups(path []byte, flushfn NodeFlushFn, rem
 	if bt.left != nil {
 		switch n := bt.left.(type) {
 		case *InternalNode:
-			if err := n.collectChildGroups(appendBit(path, 0), flushfn, remainingLevels-1); err != nil {
+			if err := n.collectChildGroups(appendBit(path, 0), flushfn, groupDepth, remainingLevels-1); err != nil {
 				return err
 			}
 		default:
 			// StemNode, HashedNode, or Empty - they handle their own collection
-			if err := bt.left.CollectNodes(appendBit(path, 0), flushfn); err != nil {
+			if err := bt.left.CollectNodes(appendBit(path, 0), flushfn, groupDepth); err != nil {
 				return err
 			}
 		}
@@ -239,12 +242,12 @@ func (bt *InternalNode) collectChildGroups(path []byte, flushfn NodeFlushFn, rem
 	if bt.right != nil {
 		switch n := bt.right.(type) {
 		case *InternalNode:
-			if err := n.collectChildGroups(appendBit(path, 1), flushfn, remainingLevels-1); err != nil {
+			if err := n.collectChildGroups(appendBit(path, 1), flushfn, groupDepth, remainingLevels-1); err != nil {
 				return err
 			}
 		default:
 			// StemNode, HashedNode, or Empty - they handle their own collection
-			if err := bt.right.CollectNodes(appendBit(path, 1), flushfn); err != nil {
+			if err := bt.right.CollectNodes(appendBit(path, 1), flushfn, groupDepth); err != nil {
 				return err
 			}
 		}
diff --git a/trie/bintrie/internal_node_test.go b/trie/bintrie/internal_node_test.go
index 158d8b7147..c8173fd69e 100644
--- a/trie/bintrie/internal_node_test.go
+++ b/trie/bintrie/internal_node_test.go
@@ -95,7 +95,7 @@ func TestInternalNodeGetWithResolver(t *testing.T) {
 				Values: values[:],
 				depth:  1,
 			}
-			return SerializeNode(stemNode), nil
+			return SerializeNode(stemNode, MaxGroupDepth), nil
 		}
 		return nil, errors.New("node not found")
 	}
@@ -379,7 +379,7 @@ func TestInternalNodeCollectNodes(t *testing.T) {
 		collectedNodes = append(collectedNodes, n)
 	}
 
-	err := node.CollectNodes([]byte{1}, flushFn)
+	err := node.CollectNodes([]byte{1}, flushFn, MaxGroupDepth)
 	if err != nil {
 		t.Fatalf("Failed to collect nodes: %v", err)
 	}
diff --git a/trie/bintrie/iterator.go b/trie/bintrie/iterator.go
index 9b863ed1e3..ba900b4321 100644
--- a/trie/bintrie/iterator.go
+++ b/trie/bintrie/iterator.go
@@ -184,7 +184,7 @@ func (it *binaryNodeIterator) Path() []byte {
 
 // NodeBlob returns the serialized bytes of the current node.
 func (it *binaryNodeIterator) NodeBlob() []byte {
-	return SerializeNode(it.current)
+	return SerializeNode(it.current, it.trie.groupDepth)
 }
 
 // Leaf returns true iff the current node is a leaf node.
diff --git a/trie/bintrie/stem_node.go b/trie/bintrie/stem_node.go
index 60856b42ce..5b7fd56e27 100644
--- a/trie/bintrie/stem_node.go
+++ b/trie/bintrie/stem_node.go
@@ -134,8 +134,8 @@ func (bt *StemNode) Hash() common.Hash {
 }
 
 // CollectNodes collects all child nodes at a given path, and flushes it
-// into the provided node collector.
-func (bt *StemNode) CollectNodes(path []byte, flush NodeFlushFn) error {
+// into the provided node collector. groupDepth is ignored for StemNodes.
+func (bt *StemNode) CollectNodes(path []byte, flush NodeFlushFn, groupDepth int) error {
 	flush(path, bt)
 	return nil
 }
diff --git a/trie/bintrie/stem_node_test.go b/trie/bintrie/stem_node_test.go
index d8d6844427..595fb7b4f3 100644
--- a/trie/bintrie/stem_node_test.go
+++ b/trie/bintrie/stem_node_test.go
@@ -347,7 +347,7 @@ func TestStemNodeCollectNodes(t *testing.T) {
 		collectedNodes = append(collectedNodes, n)
 	}
 
-	err := node.CollectNodes([]byte{0, 1, 0}, flushFn)
+	err := node.CollectNodes([]byte{0, 1, 0}, flushFn, MaxGroupDepth)
 	if err != nil {
 		t.Fatalf("Failed to collect nodes: %v", err)
 	}
diff --git a/trie/bintrie/trie.go b/trie/bintrie/trie.go
index c082d57bdf..5faec691b1 100644
--- a/trie/bintrie/trie.go
+++ b/trie/bintrie/trie.go
@@ -115,9 +115,10 @@ func NewBinaryNode() BinaryNode {
 
 // BinaryTrie is the implementation of https://eips.ethereum.org/EIPS/eip-7864.
 type BinaryTrie struct {
-	root   BinaryNode
-	reader *trie.Reader
-	tracer *trie.PrevalueTracer
+	root       BinaryNode
+	reader     *trie.Reader
+	tracer     *trie.PrevalueTracer
+	groupDepth int // Number of levels per serialized group (1-8, default 8)
 }
 
 // ToDot converts the binary trie to a DOT language representation. Useful for debugging.
@@ -127,15 +128,20 @@ func (t *BinaryTrie) ToDot() string {
 }
 
 // NewBinaryTrie creates a new binary trie.
-func NewBinaryTrie(root common.Hash, db database.NodeDatabase) (*BinaryTrie, error) {
+// groupDepth specifies the number of levels per serialized group (1-8).
+func NewBinaryTrie(root common.Hash, db database.NodeDatabase, groupDepth int) (*BinaryTrie, error) {
+	if groupDepth < 1 || groupDepth > MaxGroupDepth {
+		groupDepth = MaxGroupDepth // Default to 8
+	}
 	reader, err := trie.NewReader(root, common.Hash{}, db)
 	if err != nil {
 		return nil, err
 	}
 	t := &BinaryTrie{
-		root:   NewBinaryNode(),
-		reader: reader,
-		tracer: trie.NewPrevalueTracer(),
+		root:       NewBinaryNode(),
+		reader:     reader,
+		tracer:     trie.NewPrevalueTracer(),
+		groupDepth: groupDepth,
 	}
 	// Parse the root node if it's not empty
 	if root != types.EmptyBinaryHash && root != types.EmptyRootHash {
@@ -325,9 +331,9 @@ func (t *BinaryTrie) Commit(_ bool) (common.Hash, *trienode.NodeSet) {
 
 	// The root can be any type of BinaryNode (InternalNode, StemNode, etc.)
 	err := t.root.CollectNodes(nil, func(path []byte, node BinaryNode) {
-		serialized := SerializeNode(node)
+		serialized := SerializeNode(node, t.groupDepth)
 		nodeset.AddNode(path, trienode.NewNodeWithPrev(node.Hash(), serialized, t.tracer.Get(path)))
-	})
+	}, t.groupDepth)
 	if err != nil {
 		panic(fmt.Errorf("CollectNodes failed: %v", err))
 	}
@@ -355,9 +361,10 @@ func (t *BinaryTrie) Prove(key []byte, proofDb ethdb.KeyValueWriter) error {
 // Copy creates a deep copy of the trie.
 func (t *BinaryTrie) Copy() *BinaryTrie {
 	return &BinaryTrie{
-		root:   t.root.Copy(),
-		reader: t.reader,
-		tracer: t.tracer.Copy(),
+		root:       t.root.Copy(),
+		reader:     t.reader,
+		tracer:     t.tracer.Copy(),
+		groupDepth: t.groupDepth,
 	}
 }
 
diff --git a/triedb/database.go b/triedb/database.go
index e7e47bb91a..5ea3c62e6c 100644
--- a/triedb/database.go
+++ b/triedb/database.go
@@ -31,10 +31,11 @@ import (
 
 // Config defines all necessary options for database.
 type Config struct {
-	Preimages bool           // Flag whether the preimage of node key is recorded
-	IsVerkle  bool           // Flag whether the db is holding a verkle tree
-	HashDB    *hashdb.Config // Configs for hash-based scheme
-	PathDB    *pathdb.Config // Configs for experimental path-based scheme
+	Preimages        bool           // Flag whether the preimage of node key is recorded
+	IsVerkle         bool           // Flag whether the db is holding a verkle tree
+	BinTrieGroupDepth int           // Number of levels per serialized group in binary trie (1-8, default 8)
+	HashDB           *hashdb.Config // Configs for hash-based scheme
+	PathDB           *pathdb.Config // Configs for experimental path-based scheme
 }
 
 // HashDefaults represents a config for using hash-based scheme with
@@ -48,9 +49,10 @@ var HashDefaults = &Config{
 // VerkleDefaults represents a config for holding verkle trie data
 // using path-based scheme with default settings.
 var VerkleDefaults = &Config{
-	Preimages: false,
-	IsVerkle:  true,
-	PathDB:    pathdb.Defaults,
+	Preimages:        false,
+	IsVerkle:         true,
+	BinTrieGroupDepth: 8, // Default to byte-aligned groups
+	PathDB:           pathdb.Defaults,
 }
 
 // backend defines the methods needed to access/update trie nodes in different
@@ -380,6 +382,15 @@ func (db *Database) IsVerkle() bool {
 	return db.config.IsVerkle
 }
 
+// BinTrieGroupDepth returns the group depth for binary trie serialization (1-8).
+// Returns 8 as default if not configured.
+func (db *Database) BinTrieGroupDepth() int {
+	if db.config.BinTrieGroupDepth < 1 || db.config.BinTrieGroupDepth > 8 {
+		return 8 // Default
+	}
+	return db.config.BinTrieGroupDepth
+}
+
 // Disk returns the underlying disk database.
 func (db *Database) Disk() ethdb.Database {
 	return db.disk